| - | Singles - Main Draw |
| R128 | Elina Locane (TRINE-W) def. Eleni Bougioukou (KZOO-W) 6-1, 6-1 |
| R128 | Taryn Simmers (OTTER-W) def. Ryan Morey (CALVIN) 3-6, 6-4, 10-6 |
| R128 | Vanessa Akuaku (EC-W) def. Sasha Riley (WOOW) 6-2, 6-0 |
| R128 | Katie King (DPUW) def. Abby Cunningham (ANDERSON) 4-6, 6-1, 10-7 |
| R128 | Rafaella Ramos (DENW) def. Anya Mookencherry (LFC) 6-3, 6-4 |
| R128 | Katja Carlberg (NPU) def. Ezerskis, Liveta (NCCW) 6-3, 6-1 |
| R128 | Julia Hooker (CALVIN) def. Emerson Donaldson (RHIT-W) 6-2, 6-3 |
| R128 | Ivana Stefanova (MCW) def. Kina Ehlers (FRN) 5-7, 6-3, 10-3 |
| R128 | Scarlett Stephens (CAP) def. Maddy Williams (TRANSY) 6-1, 6-0 |
| R128 | Maia Lecuona Ramos (IITWT) def. Jenny Powless (HAN) 0-6, 6-3, 10-4 |
| R128 | Trisha Vellanki (CWR-W) def. Kimbeley Peterson (PRIN) 6-1, 6-1 |
| R128 | Maya Stolarsky (KENW) def. Maggie McBratney (WEB) 6-2, 6-0 |
| R128 | Hannah Colbert (AUGIE) def. Hayley Lale (WEST) 6-1, 6-0 |
| R128 | Hana Nouaime (KENW) def. Maya Lee (KZOO-W) 6-1, 6-1 |
| R128 | Maddie Larsen (DENW) def. Lipske, Gabi (IWU) 6-4, 6-1 |
| R128 | Emily Chiasson (WASHU) def. Jamie Hurst (ONU) 6-2, 6-2 |
| R128 | Hernandez Cisneros (PRIN) def. Ramallo Serrano (WEB) 6-0, 6-1 |
| R128 | Ehrlich, Amber (IWU) def. Maddalena Dumangane (IITWT) 6-2, 6-2 |
| R128 | Ariana Rodriguez (DENW) def. Ella Frantz (ANDERSON) 6-0, 6-1 |
| R128 | Anna-Maria Huebel (AUGIE) def. Lanie Carder (GREEN) 6-4, 1-6, 10-7 |
| R128 | Maripaz Ramirez (AUGIE) def. Lawrencia Manu (EC-W) 6-3, 3-6, 10-6 |
| R128 | Herlihy, Kate (NCCW) def. Madelyn Santner (HAN) 6-2, 6-1 |
| R128 | Anna Leach (DENW) def. Elle Loch (DPUW) 6-1, 6-1 |
| R128 | Hadley Krygier (HOPE) def. Natalie Labicki (MCW) 6-2, 6-2 |
| R128 | Elizabeth Meiners (WOOW) def. Jada Barlow (WEST) 6-0, 6-0 |
| R128 | Prachi Shah (CWR-W) def. Stephanie Makris (CAP) 6-1, 6-2 |
| R128 | Maggie Shope (KENW) def. Abby Cruser (ONU) 4-6, 7-5, 10-3 |
| R128 | Valerie Doherty (DPUW) def. Molly Stevison (KZOO-W) 6-4, 6-1 |
| R128 | Daniela De Mata (OTTER-W) def. Emma Beehler (TRINE-W) 6-0, 6-2 |
| R128 | Alexandra Pugachova (NPU) def. Meredith Cooper (TRANSY) 6-0, 6-0 |
| R128 | Marina Ribeiro (CALVIN) def. Paige Mills (RHIT-W) 6-0, 6-1 |
| R128 | Claire Earley (ADRIAN-W) def. Claire Cardie (FRN) 7-6 (7-5), 4-6, 10-7 |
| R64 | Clara Zou (UCHI-W) def. Elina Locane (TRINE-W) 6-0, 6-0 |
| R64 | Claire Hudson (KENW) def. Taryn Simmers (OTTER-W) 6-0, 6-0 |
| R64 | Lily Brecknock (WASHU) def. Vanessa Akuaku (EC-W) 6-1, 6-0 |
| R64 | Jenna Bell (ONU) def. Katie King (DPUW) 4-6, 6-1, 10-7 |
| R64 | Caitlin Bui (WASHU) def. Rafaella Ramos (DENW) 6-4, 6-2 |
| R64 | Katie Wong (CWR-W) def. Katja Carlberg (NPU) 6-2, 6-2 |
| R64 | Amanda Lopykinski (HOPE) def. Julia Hooker (CALVIN) 6-1, 6-0 |
| R64 | Sarena Biria (UCHI-W) def. Ivana Stefanova (MCW) 6-0, 6-1 |
| R64 | Eleanor Archer (WASHU) def. Scarlett Stephens (CAP) 6-3, 6-1 |
| R64 | Hye Jin Elliot (ADRIAN-W) def. Maia Lecuona Ramos (IITWT) 6-1, 6-1 |
| R64 | Anika Paul (UCHI-W) def. Trisha Vellanki (CWR-W) 6-4, 6-4 |
| R64 | Peyton Oda (DENW) def. Maya Stolarsky (KENW) 6-0, 4-6, 10-3 |
| R64 | Penados, Abigail (NCCW) def. Hannah Colbert (AUGIE) 6-2, 6-4 |
| R64 | Oliwia Mikos (UCHI-W) def. Hana Nouaime (KENW) 6-3, 6-2 |
| R64 | Katalina Wang (CWR-W) def. Maddie Larsen (DENW) 6-3, 6-3 |
| R64 | Emily Chiasson (WASHU) def. Sofia Strupovets (LFC) 1-6, 6-3, 10-7 |
| R64 | Addison Lyons (KZOO-W) def. Hernandez Cisneros (PRIN) 6-0, 6-1 |
| R64 | Kiara Komlodi (KENW) def. Ehrlich, Amber (IWU) 6-0, 6-1 |
| R64 | Lilli Dockal (LFC) def. Ariana Rodriguez (DENW) 6-2, 6-1 |
| R64 | Tina Kintiroglou (UCHI-W) def. Anna-Maria Huebel (AUGIE) 6-2, 6-1 |
| R64 | Lily Zawenah (UCHI-W) def. Maripaz Ramirez (AUGIE) 6-1, 6-2 |
| R64 | Lauren Okrainec (ONU) def. Anna Leach (DENW) 6-4, 6-4 |
| R64 | Ally Lin (WASHU) def. Madison Wei (HOPE) 6-1, 6-1 |
| R64 | Hadley Krygier (HOPE) def. Elizabeth Meiners (WOOW) 6-1, 6-7 (3-7), 11-9 |
| R64 | Isabelle Chong (UCHI-W) def. Prachi Shah (CWR-W) 6-0, 6-0 |
| R64 | Maggie Shope (KENW) def. Caitlin VanWinkle (DENW) 6-3, 6-1 |
| R64 | Eliana Hanna (WASHU) def. Valerie Doherty (DPUW) 6-0, 6-2 |
| R64 | Tara Markovic (CWR-W) def. Daniela De Mata (OTTER-W) 6-4, 6-2 |
| R64 | Amber Edmonds (WASHU) def. Alexandra Pugachova (NPU) 7-6 (7-2), 7-6 (7-3) |
| R64 | Payne, Kensington (NCCW) def. Marina Ribeiro (CALVIN) 6-1, 6-1 |
| R64 | Eleni Lazaridou (KENW) def. Claire Earley (ADRIAN-W) 6-0, 6-0 |
| R32 | Clara Zou (UCHI-W) def. Claire Hudson (KENW) 6-3, 6-1 |
| R32 | Lily Brecknock (WASHU) def. Jenna Bell (ONU) 7-5, 6-1 |
| R32 | Caitlin Bui (WASHU) def. Katie Wong (CWR-W) 6-1, 6-0 |
| R32 | Sarena Biria (UCHI-W) def. Amanda Lopykinski (HOPE) 6-0, 6-1 |
| R32 | Eleanor Archer (WASHU) def. Hye Jin Elliot (ADRIAN-W) walkover |
| R32 | Anika Paul (UCHI-W) def. Peyton Oda (DENW) 6-2, 6-2 |
| R32 | Penados, Abigail (NCCW) def. Oliwia Mikos (UCHI-W) 6-2, 7-6 (7-5) |
| R32 | Katalina Wang (CWR-W) def. Emily Chiasson (WASHU) 7-5, 7-6 (7-5) |
| R32 | Kiara Komlodi (KENW) def. Addison Lyons (KZOO-W) 6-3, 6-3 |
| R32 | Tina Kintiroglou (UCHI-W) def. Lilli Dockal (LFC) 6-3, 6-1 |
| R32 | Lily Zawenah (UCHI-W) def. Atra Biria (CWR-W) 6-2, 6-3 |
| R32 | Ally Lin (WASHU) def. Lauren Okrainec (ONU) 6-3, 6-2 |
| R32 | Isabelle Chong (UCHI-W) def. Hadley Krygier (HOPE) 7-5, 4-6, 10-2 |
| R32 | Eliana Hanna (WASHU) def. Maggie Shope (KENW) 6-0, 6-0 |
| R32 | Amber Edmonds (WASHU) def. Tara Markovic (CWR-W) 7-5, 6-4 |
| R32 | Eleni Lazaridou (KENW) def. Payne, Kensington (NCCW) 6-1, 6-2 |
| R16 | Lily Brecknock (WASHU) def. Clara Zou (UCHI-W) 6-4, 2-6, 6-2 |
| R16 | Sarena Biria (UCHI-W) def. Caitlin Bui (WASHU) 6-3, 3-6, 6-4 |
| R16 | Anika Paul (UCHI-W) def. Eleanor Archer (WASHU) 7-6 (7-5), 6-3 |
| R16 | Katalina Wang (CWR-W) def. Penados, Abigail (NCCW) 0-6, 6-3, 6-2 |
| R16 | Kiara Komlodi (KENW) def. Tina Kintiroglou (UCHI-W) 6-3, 6-4 |
| R16 | Ally Lin (WASHU) def. Lily Zawenah (UCHI-W) 6-2, 2-6, 7-6 (10-8) |
| R16 | Eliana Hanna (WASHU) def. Isabelle Chong (UCHI-W) 6-1, 6-2 |
| R16 | Eleni Lazaridou (KENW) def. Amber Edmonds (WASHU) 6-0, 6-1 |
| QF | Sarena Biria (UCHI-W) def. Lily Brecknock (WASHU) 7-5, 6-1 |
| QF | Anika Paul (UCHI-W) def. Katalina Wang (CWR-W) 7-6 (7-2), 6-4 |
| QF | Kiara Komlodi (KENW) def. Ally Lin (WASHU) 6-2, 7-6 (7-5) |
| QF | Eliana Hanna (WASHU) def. Eleni Lazaridou (KENW) 7-6 (9-7), 6-2 |
| SF | Sarena Biria (UCHI-W) def. Anika Paul (UCHI-W) 6-1, 2-6, 6-3 |
| SF | Eliana Hanna (WASHU) def. Kiara Komlodi (KENW) 7-5, 6-3 |
| F | Sarena Biria (UCHI-W) def. Eliana Hanna (WASHU) 2-6, 6-2, 6-4 |
